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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was observed to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a vital role in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are described. Two supplemental splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however readily available.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.
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This guideline outlines more feasible optimization of mobile and immunoassay parameters to be certain the absolute best final results are...
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This manual outlines more attainable optimization of mobile and immunoassay parameters to be sure the absolute best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each mitotic party. Cyclin E types a complex with and capabilities to be a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S phase boundary which is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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